AffiAB® Anti-p21 ARC Antibody
Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (. The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility . In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA . The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) .
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: O15145 Human; SwissProt: Q9JM76 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human p21 ARC aa 120-178.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
衰老
Recommended dilutions
WB: 1:500-1:2, 000; IHC-P: 1:50-1:200; IF-Cell: 1:50-1:100
Molecular Weight
21 kDa
Subcellular location
Cell projection, Cytoplasm, Cytoskeleton, Nucleus.
Positive control
THP-1 cell lysate, rat lung tissue lysate, mouse lung tissue lysate, rat brain tissue, human colon tissue, HUVEC cell lysate.