AffiAB® Anti-OTC/Ornithine Carbamoyltransferase Antibody
Ornithine transcarbamylase (OTC) (also called ornithine carbamoyltransferase) is an enzyme (EC 2.1.3.3) that catalyzes the reaction between carbamoyl phosphate (CP) and ornithine (Orn) to form citrulline (Cit) and phosphate (Pi) . There are two classes of OTC anabolic and catabolic. This article focuses on anabolic OTC. Anabolic OTC facilitates the sixth step in the biosynthesis of the amino acid arginine in prokaryotes. [5] In contrast, mammalian OTC plays an essential role in the urea cycle whose purpose is to capture toxic ammonia and transform it into less toxic urea nitrogen source for excretion.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P00480 Human; SwissProt: P11725 Mouse; SwissProt: P00481 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human OTC/Ornithine Carbamoyltransferase aa 200-354.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:5, 000; IF-Cell: 1:50-1:200; IHC-P: 1:100-1:500
Molecular Weight
40 kDa
Subcellular location
Mitochondrion.
Positive control
Rat colon tissue lysate, human liver tissue lysate, mouse liver tissue lysate, SW480, human liver carcinoma tissue, human liver tissue.