AffiAB® Anti-Osteopontin Antibody
Osteopontin is an extracellular matrix cell adhesion protein which is abundant in bone and synthesized by preosteoblasts, osteoblasts and osteoclastic cells that are localized in the mineralized phase of bone matrix. Osteopontin probably exists in tissue-specific isoforms that may correspond to particular cellular functions. Acts as a cytokine it involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P10451 Human; SwissProt: P10923 Mouse; SwissProt: P08721 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within mouse Osteopontin aa 245-294 / 294.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Cancer, PI3K-AKT
Recommended dilutions
WB: 1:500-1:2, 000
Molecular Weight
Predicted band size: 35 kDa
Subcellular location
Secreted.
Positive control
Rat bone marrow tissue lysates, mouse kidney tissue lysate, rat kidney tissue lysate, MCF-7 cell lysates.