![AffiAB® Anti-Oct4 Antibody [A3-C11]](/web/image/product.product/77276/image_1024/%5BAFG-HB-2786%5D%20AffiAB%C2%AE%20Anti-Oct4%20Antibody%20%5BA3-C11%5D%20?unique=b9b0f1e)
AffiAB® Anti-Oct4 Antibody [A3-C11]
Oct-4 (POU5F1) is transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') . It forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Oct-4 is critical for early embryogenesis and for embryonic stem cell pluripotency. It is expressed in developing brain and highest levels also found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: Q01860 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG1
Clone
A3-C11
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell
Predicted species reactivity
N/A
Immunogen
Recombinant protein within full length human Oct4 protein.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:5, 000; IF-Cell: 1:200
Molecular Weight
Predicted band size: 39 kDa
Subcellular location
Nucleus
Positive control
NCCIT cell lysate, D3 cell lysate, NCCIT.