![AffiAB® Anti-NUP50 Antibody [JE64-06]](/web/image/product.product/77265/image_1024/%5BAFG-HB-2774%5D%20AffiAB%C2%AE%20Anti-NUP50%20Antibody%20%5BJE64-06%5D%20?unique=b83e256)
AffiAB® Anti-NUP50 Antibody [JE64-06]
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q9UKX7 Human; SwissProt: Q9JIH2 Mouse; SwissProt: O08587 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JE64-06
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human NUP50 aa 11-110/468.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500
; IHC-P: 1:100-1:400
Molecular Weight
Predicted band size: 50 kDa
Subcellular location
Nuclear pore complex, Nucleus membrane.
Positive control
Jurkat cell lysate, Hela cell lysate, HepG2 cell lysate, rat large intestine tissue, human liver carcinoma tissue, human colon carcinoma tissue, mouse hippocampus tissue, mouse brain tissue, rat testis tissue lysate, mouse testis tissue lysate.