AffiAB® Anti-Nucleolin Antibody
Nucleolin is a multi-functional protein that is one of the major components of the nucleoli. It plays an essential role in various steps of ribosome biogenesis including rRNA synthesis, processing of pre-rRNA, pre-ribosomal RNA assembly, and transport of ribosomal proteins out of the nucleus. While the main function of nucleolin is ribosome biogenesis, it plays an important role in various other nuclear activities. Down regulation of nucleolin leads to increased expression of p53, defects in genome duplication, and a delay at prometaphase during mitosis leading to cell cycle arrest.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P19338 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Nucleolin aa 201-250 / 710.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000; IF-Cell: 1:100-1:200
Molecular Weight
~100kDa
Subcellular location
Cell nucleus, cytoplasm
Positive control
K562, Hela, Jurkat, 293T, mouse embryonic stem cell