![AffiAB® Anti-NSUN4 Antibody [4G4]](/web/image/product.product/77245/image_1024/%5BAFG-HB-2753%5D%20AffiAB%C2%AE%20Anti-NSUN4%20Antibody%20%5B4G4%5D%20?unique=b83e256)
AffiAB® Anti-NSUN4 Antibody [4G4]
Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU) . Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: Q96CB9 Human; SwissProt: Q9CZ57 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG1
Clone
4G4
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human NSUN4 aa 1-384.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein G affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:1, 000; IHC-P: 1:100-1:500
Molecular Weight
43 kDa
Subcellular location
Mitochondrion.
Positive control
MCF-7 cell lysate, 293 cell lysate, mouse testis tissue, human prostate tissue.