AffiAB® Anti-NSE Antibody [SC06-28]
Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE) , is an enzyme that in humans is encoded by the ENO2 gene. Gamma-enolase is a phosphopyruvate hydratase. Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P09104 Human; SwissProt: P17183 Mouse; SwissProt: P07323 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SC06-28
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
Zebrafish
Immunogen
Synthetic peptide within human NSE aa 30-70.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
HIF-1 signaling pathway, Neuroscience, Cancer
Recommended dilutions
WB: 1:1, 000-1:2, 000; IF-Cell: 1:50-1:100; IHC-P: 1:50-1:200
Molecular Weight
Predicted band size: 47 kDa
Subcellular location
Cytoplasm, Cell membrane.
Positive control
HepG2 cell lysate, Hela cell lysate, 293 cell lysate, SH-SY5Y, 293, rat brain tissue, mouse brain tissue.