AffiAB® Anti-NSE Antibody
Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. Detection of NSE with antibodies can be used to identify neuronal cells and cells with neuroendocrine differentiation. NSE is produced by small cell carcinomas which are neuroendocrine in origin. NSE is therefore a useful tumor marker for lung cancer patients.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P09104 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat, Zebrafish, Hybrid fish (crucian-carp)
Tested applications
WB, IF-Cell
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within N-terminal human NSE.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
HIF-1 signaling pathway, Neuroscience, Cancer
Recommended dilutions
WB: 1:2000; IF-Cell: 1:100
Molecular Weight
47 kDa
Subcellular location
Cytoplasm, cell membrane
Positive control
Hela, NIH/3T3, F9, human brain, mouse brain, A172, SHG-44, N2A hybrid fish (crucian-carp) brain tissue lysate.