AffiAB® Anti-NPHS2 Antibody [JB51-33]
Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q9NP85 Human; SwissProt: Q91X05 Mouse; SwissProt: Q8K4G9 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JB51-33
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P, FC, IF-Tissue
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human NPHS2 aa 334-383 / 383.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:1, 000; IHC-P: 1:50-1:200; FC: 1:50-1:100; IF-Tissue: 1:100
Molecular Weight
Predicted band size: 42 kDa
Subcellular location
Cell membrane. Endoplasmic reticulum.
Positive control
Rat kidney tissue lysate, human kidney tissue lysate, rat kidney tissue, human kidney tissue, mouse kidney tissue, 293T.