AffiAB® Anti-Nodal Antibody
Nodal is a secretory protein that in humans is encoded by the NODAL genewhich is located on chromosome 10q22.1. It belongs to the transforming growth factor beta (TGF-β) superfamily. Like many other members of this superfamily it is involved in cell differentiation in early embryogenesis, playing a key role in signal transfer from the node, in the anterior primitive streak, to lateral plate mesoderm (LPM) .Nodal signaling is important very early in development for mesoderm and endoderm formation and subsequent organization of left-right axial structures.In addition, Nodal seems to have important functions in neural patterning, stem cell maintenanceand many other developmental processes, including left/right handedness.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q96S42 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Rat
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human Nodal aa 150-347.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:1, 000; IHC-P: 1:50-1:200
Molecular Weight
Predicted band size: 40 kDa.
Subcellular location
Secreted.
Positive control
Rat testis tissue cell lysate, SHSY5Y cell lysate, rat testis tissue, human colon tissue.