AffiAB® Anti-NHE-1 Antibody
The sodium-hydrogen antiporter 1 (NHE-1) also known as sodium/hydrogen exchanger 1 or SLC9A1 (SoLute Carrier family 9A1) is an isoform of sodium–hydrogen antiporter that in humans is encoded by the SLC9A1 gene.The Na+/H+ antiporter (SLC9A1) is a ubiquitous membrane-bound enzyme involved in volume- and pH-regulation of vertebrate cells. It is inhibited by the non-specific diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others.Sodium–hydrogen antiporter 1 has been shown to interact with carbonic anhydrase II and CHP. It is also the target of the experimental drug rimeporide, which is being developed for the treatment of Duchenne muscular dystrophy.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P19634 Human; SwissProt: Q61165 Mouse; SwissProt: P26431 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P, IF-Cell, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within mouse NHE-1 aa 36-85 / 820.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
cAMP signaling pathway
Recommended dilutions
WB: 1:500-1:1000; IHC-P: 1:50-1:200; IF-Cell: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
Predicted band size: 91/61 kDa.
Subcellular location
Cell membrane, Endoplasmic reticulum, Membrane.
Positive control
Rat skin lysates, A431, 293T, LOVO, human kidney tissue, mouse colon tissue, human small intestine tissue.