![AffiAB® Anti-NeuN Antibody [PD01-45]](/web/image/product.product/77163/image_1024/%5BAFG-HB-2662%5D%20AffiAB%C2%AE%20Anti-NeuN%20Antibody%20%5BPD01-45%5D%20?unique=b83e256)
AffiAB® Anti-NeuN Antibody [PD01-45]
Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.
Antibody type
Recombinant Mouse monoclonal Antibody
Uniprot ID
SwissProt: A6NFN3 Human; SwissProt: Q8BIF2 Mouse; Unigene:143966 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG1
Clone
PD01-45
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human NeuN aa 20-60.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
PBS (pH7.4) , 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000; IF-Cell: 1:50; IF-Tissue: 1:50; IHC-P: 1:1, 000
Molecular Weight
Predicted band size: 34 kDa
Subcellular location
Cytoplasm, Nucleus
Positive control
Human brain tissue, mouse hippocampus tissue, mouse brain tissue, mouse cerebellum tissue, rat hippocampus tissue, rat brain tissue, rat cerebellum tissue, mouse cerebellum tissue lysates.