AffiAB® Anti-NDUFB8 Antibody [JG61-36]
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) , that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: O95169 Human; SwissProt: Q9D6J5 Mouse
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JG61-36
KO/KD
N/A
Species reactivity
Human, Rat
Tested applications
WB, IP, IHC-P, FC, IF-Cell
Predicted species reactivity
Mouse
Immunogen
Recombinant protein within Human NDUFB8 aa 1-186 / 186.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IP: 1:10-1:50; IHC-P: 1:50-1:200; FC: 1:50-1:100; IF-Cell: 1:50
Molecular Weight
Predicted band size: 22 kDa
Subcellular location
Mitochondrion.
Positive control
293 cell lysate, A549 cell lysate, rat spleen tissue lysate, Hela cell lysate, rat heart tissue, human liver carcinoma tissue, human kidney tissue, human small intestine tissue, Hela.