AffiAB® Anti-NDUFB4 Antibody [JE65-30]

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CAT# AFG-HB-2638
Size: 100 μL

496.50 496.5 USD 496.50

496.50

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AffiAB® Anti-NDUFB4 Antibody [JE65-30]

NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa is a protein that in humans is encoded by the NDUFB4 gene. The NDUFB4 protein is a subunit of NADH dehydrogenase (ubiquinone) , which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. The human NDUFB4 gene codes for a subunit of Complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. However, NDUFB4 is an accessory subunit of the complex that is believed not to be involved in catalysis. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein complex has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ) , which is reduced to ubiquinol (CoQH2) . The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: O95168 Human; SwissProt: Q9CQC7 Mouse; Entrez Gene: 288088 Rat

Recombinant

YES

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

JE65-30

KO/KD

N/A

Species reactivity

Human, Mouse, Rat

Tested applications

WB, IHC-P

Predicted species reactivity

N/A

Immunogen

Recombinant protein within human NDUFB4 aa 1-129/129.

Storage

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

N/A

Recommended dilutions

WB: 1:500 ; IHC-P: 1:400

Molecular Weight

Predicted band size: 15 kDa

Subcellular location

Mitochondrion inner membrane.

Positive control

Mouse heart tissue lysates, rat heart tissue lysate, rat kidney tissue lysate, rat brain tissue lysate, human kidney tissue.