AffiAB® Anti-NCK1 Antibody [JU41-80]
The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKα) and NCK2 (NCKβ) , which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of Nck1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr 1009) ; overexpression of Nck2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2. The human NCK1 and NCK2 genes map to chromosomes 3q22.3 and 2q12.2, respectively.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P16333 Human; SwissProt: Q99M51 Mouse; SwissProt: B2RZ33 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JU41-80
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human NCK1 aa 204-296 / 377.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Immunology & Inflammation, ErbB signaling pathway
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: 1:10-1:50
Molecular Weight
43 kDa
Subcellular location
Cytoplasm. Endoplasmic reticulum. Nucleus.
Positive control
Hela cell lysate, NIH/3T3 cell lysate, rat kidney tissue lysate, mouse testis tissue lysate, LOVO, A431, HUVEC, mouse testis tissue, rat testis tissue, human tonsil tissue, human kidney tissue, Jurkat.