AffiAB® Anti-Myoglobin Antibody [SC06-38]
Myoglobin is a cytosolic oxygen binding protein responsible for the storage and diffusion of oxygen within myocytes. Expression of myoglobin is highest in skeletal and cardiac muscle. Myoglobin is necessary for the maintenance of mitochondrial respiration during heavy and sustained contractile activity, and it is thought to transport oxygen from erythroyctes to mitochondria. The genomic structure of myoglobin appears to be conserved across a broad range of species, and contains a putative polyadenylation signal and a polypyrimidine-rich region. Human myoglobin is specified by a single gene, and it has been identified in human smooth muscle.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P02144 Human; SwissProt: P04247 Mouse; SwissProt: Q9QZ76 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SC06-38
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Myoglobin aa 121-154 / 154.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Cardiovascular
Recommended dilutions
WB: 1:1, 000-1:5, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 17 kDa
Subcellular location
Cytoplasm.
Positive control
Human skeletal muscle tissue lysates, C2C12, rat skeletal muscle tissue, human fetal skeletal muscle tissue, mouse skeletal muscle tissue, mouse heart tissue.