AffiAB® Anti-Myelin PLP Antibody
Proteolipid protein 1 (PLP1) is a form of myelin proteolipid protein (PLP) . Mutations in PLP1 are associated with Pelizaeus–Merzbacher disease. It is a 4 transmembrane domain protein which is proposed to bind other copies of itself on the extracellular side of the membrane. In a myelin sheath, as the layers of myelin wraps come together, PLP will bind itself and tightly hold the cellular membranes together. This gene encodes a transmembrane proteolipid protein that is the predominant myelin protein present in the central nervous system (CNS) . The encoded protein functions in myelination. This protein may play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. Mutations associated with this gene cause X-linked Pelizaeus–Merzbacher disease and spastic paraplegia type 2. Two transcript variants encoding distinct isoforms have been identified for this gene. In melanocytic cells PLP1 gene expression may be regulated by MITF.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P60201 Human; SwissProt: P60202 Mouse; SwissProt: P60203 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within huamn PLP1 aa 37-63 (Extracellular domain) .
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Neuroscience
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:100; IHC-P: 1:50-1:200
Molecular Weight
Predicted Molecular Wt. 30 kDa
Subcellular location
Cell membrane, Myelin membrane.
Positive control
HepG2 cell lysate, mouse cerebellum tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, F9 cell, rat brain tissue, mouse brain tissue.