AffiAB® Anti-Muc1 Antibody
The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P15941 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide immune sequence is APDTRPAPGSTAPPAHGVTSC.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Cancer, Immunology & Inflammation
Recommended dilutions
WB:1:500-1:2, 000; IF-Cell:1:50-1:100; IHC-P:1:50-1:200
Molecular Weight
Predicted band size 122 kDa.
Subcellular location
Plasma membrane.
Positive control
MCF-7 cell lysates, MCF-7, human colon tissue, human skin tissue, human breast carcinoma tissue, human kidney tissue, human uterus tissue.