![AffiAB® Anti-mtTFA Antibody [JM32-44]](/web/image/product.product/77057/image_1024/%5BAFG-HB-2554%5D%20AffiAB%C2%AE%20Anti-mtTFA%20Antibody%20%5BJM32-44%5D%20?unique=a48b62f)
AffiAB® Anti-mtTFA Antibody [JM32-44]
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q00059 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JM32-44
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human mtTFA aa 41-90 / 246.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Huntington's disease, Cardiovascular
Recommended dilutions
WB: 1:200-1:500; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 29 kDa
Subcellular location
Mitochondrion, Mitochondrion nucleoid.
Positive control
Hela cell lysate, Daudi cell lysate, HL-60 cell lysate, K562 cell lysate, Hela, MCF-7, SK-Br-3, human colon carcinoma tissue, human breast tissue, human kidney tissue.