AffiAB® Anti-MTHFD2 Antibody
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial is an enzyme that in humans is encoded by the MTHFD2 gene. This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P13995 Human; SwissProt: Q9H903 Human; SwissProt: D3YZG8 Mouse; SwissProt: P18155 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human MTHFD2 aa 210-250.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB:1:500-1:2, 000
; IHC-P:1:50-1:200
; FC:1:50-1:100
Molecular Weight
38 kDa
Subcellular location
Mitochondrion.
Positive control
HL-60 cell lysate, 293T cell lysate, human colon carcinoma tissue, mouse kidney tissue, human tonsil carcinoma tissue, Daudi.