AffiAB® Anti-MKRN2 Antibody
MKRN2 gene encodes a probable E3 ubiquitin ligase containing several zinc finger domains, that is a member of the makorin RING zinc-finger protein family. This gene overlaps the v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1) gene in an antisense orientation and may have a co-regulatory function with RAF1. Alternative splicing results in multiple transcript variants. E3 ubiquitin ligase catalyze the covalent attachment of ubiquitin moieties onto substrate proteins.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q9H000 Human; SwissProt: Q9ERV1 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide corresponding to Human MRKN2 aa 281-330 / 416.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:200-1:500; IF-Cell: 1:50; IHC-P: 1:200-1:500
Molecular Weight
Predicted band size: 47kDa
Subcellular location
Cytoplasm, nucleus
Positive control
HepG2, Jurkat, 293T, mouse liver tissue, Hela, human liver tisse, mouse thymus tissue, human colorectal carcinomas tissue