AffiAB® Anti-MGMT Antibody
MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O (6) position of guanine, restoring guanine to its normal form without causing DNA breaks. MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents. It is ubiquitously expressed in normal human tissues and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P16455 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within residues of MGMT aa 158-207 / 207.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:200; IHC-P: 1:200; FC:1:100
Molecular Weight
Predicted band size: 22 kDa
Subcellular location
Nucleus.
Positive control
MCF-7 cell lysate, Hela cell lysate, Dauai cell lysate, Raji cell lysate, SK-BR-3 cell lysate, SKOV3 cell lysate, human kidney tissue lysate, human liver tissue lysate, human thymus tissue lysate, human placentae tissue lysate, A172, SK-BR-3, MCF-7, human tonsil tissue, human colon carcinoma tissue.