![AffiAB® Anti-MGEA5 Antibody [JG40-05]](/web/image/product.product/76972/image_1024/%5BAFG-HB-2463%5D%20AffiAB%C2%AE%20Anti-MGEA5%20Antibody%20%5BJG40-05%5D%20?unique=a48b62f)
AffiAB® Anti-MGEA5 Antibody [JG40-05]
Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) . Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) , but has about six times lower specific activity than isoform 1.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: O60502 Human; SwissProt: Q9EQQ9 Mouse
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JG40-05
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P, IP
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human MGEA5 aa 2-103 / 916.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000
; IHC-P: 1:50-1:200
; IP: 1:10-1:50
Molecular Weight
103 kDa
Subcellular location
Cytoplasm. Nucleus.
Positive control
PC-3M cell lysate, SH-SY5Y cell lysate, human tonsil tissue, human pancreas tissue, mouse testis tissue.