AffiAB® Anti-MEMO1 Antibody
May control cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Mediator of ERBB2 signaling. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Is required for breast carcinoma cell migration.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q9Y316 Human; SwissProt: Q91VH6 Mouse; SwissProt: Q4QQR9 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human MEMO1 aa 1-200.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:2, 000; IHC-P: 1:100-1:500
Molecular Weight
Predicted band size: 34 kDa.
Subcellular location
Cytosol, nucleus.
Positive control
Mouse lung tissue lysate, mouse colon tissue lysate, mouse liver tissue, human skeletal muscle tissue, rat large intestine tissue.