AffiAB® Anti-MED4 Antibody [JE56-55]
This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q9NPJ6 Human; SwissProt: Q3UL96 Mouse; SwissProt: Q8BVG6 Mouse; SwissProt: Q9CQA5 Mouse; SwissProt: Q561Q8 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JE56-55
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human MED4 aa 100-250.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000
; IHC-P: 1:50-1:200
Molecular Weight
Predicted band size: 30 kDa.
Subcellular location
Nucleus.
Positive control
SH-SY5Y cell lysate, rat testis tissue lysate, mouse thymus tissue lysate, rat testis tissue, human small intestine tissue.