AffiAB® Anti-MDM2 Antibody
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q00987 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human MDM2 aa 1-50 / 491.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
p53 signaling pathway, Cancer, PI3K-AKT, 衰老
Recommended dilutions
WB:1:500-1:1, 000; IF-Cell:1:50-1:200; IHC-P:1:500-1:1, 000; FC:1:50-1:100
Molecular Weight
55 kDa
Subcellular location
Nucleoplasm, nucleolus, cytoplasm.
Positive control
Human lung tissue lysate, Daudi cell lysate, HepG2, JAR, MCF-7, human thyroid tissue, human skin tissue, human breast carcinoma tissue, human small intestine tissue, human pancreas tissue, Daudi.