AffiAB® Anti-mCherry Antibody

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CAT# AFG-HB-2413
Size: 100 μL

433.50 433.5 USD 433.50

433.50

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AffiAB® Anti-mCherry Antibody

mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs) . As a RFP, mCherry was derived from DsRed of Discosoma sea anemones unlike green fluorescent proteins (GFPs) which are often derived from Aequoera victoria jellyfish.Fluorescent proteins are used to tag components in the cell, so they can be studied using fluorescence spectroscopy and fluorescence microscopy. mCherry absorbs light between 540-590 nm and emits light in the range of 550-650 nm. mCherry belongs to the group of fluorescent protein chromophores used as instruments to visualize genes and analyze their functions in experiments. Genome editing has been improved greatly through the precise insertion of these fluorescent protein tags into the genetic material of many diverse organisms. Most comparisons between the brightness and photostability of different fluorescent proteins have been made in vitro, removed from biological variables that affect protein performance in cells or organisms. It is hard to perfectly simulate cellular environments in vitro, and the difference in environment could have an effect on the brightness and photostability.

Antibody type

Rabbit polyclonal Antibody

Uniprot ID

SwissProt: X5DSL3 AnaplasmaMarginale

Recombinant

NO

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

N/A

KO/KD

N/A

Species reactivity

N/A

Tested applications

WB, ELISA

Predicted species reactivity

N/A

Immunogen

Recombinant protein within mCherry 1-236aa.

Storage

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Immunogen affinity purified.

Signal pathway

N/A

Recommended dilutions

WB: 1:1, 000-1:2, 000 ; ELISA: 1:1, 000-1:5, 000

Molecular Weight

N/A

Subcellular location

N/A

Positive control

Recombinant mCherry protein lysates.