AffiAB® Anti-MAT2A Antibody
Protein expression is regulated by post-transcriptional regulation: in presence of S-adenosyl-L-methionine, METTL16 binds and methylates the first hairpin of the 3'-UTR region of MAT2A mRNA, impairing MAT2A expression . In S-adenosyl-L-methionine-limiting conditions, METTL16 binds the 3'-UTR region of MAT2A mRNA without methylating it due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A . Consequences of N6-methylation of MAT2A are however subject to discussion: According to a report, N6-methylation of MAT2A affects MAT2A mRNA splicing ) . According to a second report, N6-methylation of MAT2A affects MAT2A mRNA stability (By similarity) .By similarity1 Publication.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P31153 Human; SwissProt: Q3THS6 Mouse; SwissProt: P18298 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human MAT2A aa 70-260.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IHC-P: 1:500-1:2, 000; FC: 1:500-1:1, 000
Molecular Weight
44 kDa
Subcellular location
Nucleoplasm, Cytosol.
Positive control
Rat kidney tissue lysates, rat kidney tissue, human colon tissue, human colon carcinoma tissue, mouse brain tissue, Hela.