![AffiAB® Anti-Lin28B Antibody [A2-4-5]](/web/image/product.product/76829/image_1024/%5BAFG-HB-2315%5D%20AffiAB%C2%AE%20Anti-Lin28B%20Antibody%20%5BA2-4-5%5D%20?unique=315d7a9)
AffiAB® Anti-Lin28B Antibody [A2-4-5]
LIN28 is thought to regulate the self-renewal of stem cells. In nematodes, the LIN28 homolog lin-28 is a heterochronic gene that determines the onset of early larval stages of developmental events in Caenorhabditis elegans, by regulating the self-renewal of nematode stem cells in the skin (called seam cells) and vulva (called VPCs) during development. In mice, LIN28 is highly expressed in mouse embryonic stem cells and during early embryogenesis.It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7) , a miRNA precursor. Lin28B is high expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC) .
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: Q6ZN17 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG2a
Clone
A2-4-5
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human Lin28B aa 73-250 / 250.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Cancer
Recommended dilutions
WB: 1:100-1:500; IF-Cell: 1:100-1:200
Molecular Weight
~27 kDa
Subcellular location
Cytoplasm, nucleus
Positive control
NCCIT, Lin28B recombinant protein