![AffiAB® Anti-LIN28A Antibody [A4-A6]](/web/image/product.product/76828/image_1024/%5BAFG-HB-2314%5D%20AffiAB%C2%AE%20Anti-LIN28A%20Antibody%20%5BA4-A6%5D%20?unique=315d7a9)
AffiAB® Anti-LIN28A Antibody [A4-A6]
LIN28A is conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs. LIN28A is localized to the periendoplasmic reticulum (ER) area and inhibits translation of mRNAs that are destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: Q9H9Z2 Human; SwissProt: Q8K3Y3 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG2b
Clone
A4-A6
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:10, 000; IF-Cell: 1:100; IHC-P: 1:100
Molecular Weight
Predicted band size: 23 kDa
Subcellular location
Cytoplasm, Nucleus.
Positive control
NCCIT cell lysate, F9 cell, human kidney cancer tissue.