![AffiAB® Anti-Lin28 Antibody [JA10-63]](/web/image/product.product/76827/image_1024/%5BAFG-HB-2313%5D%20AffiAB%C2%AE%20Anti-Lin28%20Antibody%20%5BJA10-63%5D%20?unique=a48b62f)
AffiAB® Anti-Lin28 Antibody [JA10-63]
LIN-28 is a highly conserved, RNA-binding, cytoplasmic protein. It consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs that were first identified in Caenorhabditis elegans. LIN-28 controls the timing of events during embryonic development and is readily expressed in embryos, embryonic stem cells and embryonal carcinoma cells. The presence of LIN-28 persists in some adult tissues including cardiac and skeletal muscle. In differentiating myoblasts, LIN-28 increases protein sysnthesis efficiency and binds to the growth and differentiation factor IGF-II.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q9H9Z2 Human; SwissProt: Q8K3Y3 Mouse; SwissProt: D3ZZA6 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JA10-63
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P, IP, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Lin28 aa 10-59 / 209.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:1, 000
; IHC-P: 1:400
; IP: 1:10-1:50
; FC: 1:50-1:100
Molecular Weight
Predicted band size: 23 kDa
Subcellular location
Cytoplasm. Nucleus.
Positive control
NCCIT cell lysates, Hela, K562 cell lysates, rat testis tissue, human testis tissue, mouse placenta tissue.