AffiAB® Anti-Lamin A + Lamin C Antibody [A6F5]
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: P02545 Human; SwissProt: P48679 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG
Clone
A6F5
KO/KD
N/A
Species reactivity
Human, Rat
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human Lamin A/C aa 151-350/664.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
PBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein G affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500
; IHC-P: 1:600
Molecular Weight
Predicted band size: 74/65 kDa
Subcellular location
Nucleus, Nucleus envelope, Nucleus lamina, Nucleoplasm, Nucleus matrix; Nucleus speckle.
Positive control
Hela cell lysate, A549 cell lysate, U87MG cell lysate, human skin tissue, rat kidney tissue, rat heart tissue.