![AffiAB® Anti-Lactate Dehydrogenase Antibody [SU39-06]](/web/image/product.product/76796/image_1024/%5BAFG-HB-2279%5D%20AffiAB%C2%AE%20Anti-Lactate%20Dehydrogenase%20Antibody%20%5BSU39-06%5D%20?unique=a48b62f)
AffiAB® Anti-Lactate Dehydrogenase Antibody [SU39-06]
Lactate dehydrogenase (LDH or LD) is an enzyme found in nearly all living cells. LDH catalyzes the conversion of lactate to pyruvate and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another. LDH exists in four distinct enzyme classes. LDH is expressed extensively in body tissues, such as blood cells and heart muscle. Because it is released during tissue damage, it is a marker of common injuries and disease such as heart failure.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P07195 Human; SwissProt: P00338 Human; SwissProt: P16125 Mouse; SwissProt: P06151 Mouse; SwissProt: P42123 Rat; SwissProt: P04642 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SU39-06
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Predicted species reactivity
Rat, Zebrafish
Immunogen
Synthetic peptide within human LDHB aa 270-310.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
HIF-1 signaling pathway, Cancer
Recommended dilutions
WB: 1:1, 000-1:5, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:200-1:2, 000; FC: 1:50-1:100; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 37 kDa
Subcellular location
Cytoplasm.
Positive control
Hela cell lysate, A549 cell lysate, MCF-7 cell lysate, hybrid fish (crucian-carp) brain tissue lysates, A549, A431, human liver tissue, human liver carcinoma tissue, human breast carcinoma tissue, mouse liver tissue, mouse testis tissue, mouse skeletal muscle tissue, Hela, LOVO.