AffiAB® Anti-Kv1.1 potassium channel Antibody [SN66-06]
Voltage-gated K+ channels in the plasma membrane control the repolarization and the frequency of action potentials in neurons, muscles, and other excitable cells. The KV gene family encodes more than 30 genes that comprise the subunits of the K+ channels, and they vary in their gating and permeation properties, subcellular distribution, and expression patterns. Functional KV channels assemble as tetramers consisting of pore-forming -subunits (KVα) , which include the KV1, KV2, KV3, and KV4 proteins, and accessory or KVβ subunits that modify the gating properties of the coexpressed KVα subunits. Differences exist in the patterns of trafficking, biosynthetic processing and surface expression of the major KV1 subunits (KV1.1, KV1.2, KV1.4, KV1.5 and KV1.6) expressed in rat and human brain, suggesting that the individual protein subunits are highly regulated to control for the assembly and formation of functional neuronal channels.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q09470 Human; SwissProt: P16388 Mouse; SwissProt: P10499 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SN66-06
KO/KD
N/A
Species reactivity
Human, Rat
Tested applications
WB, IHC-P, IP
Predicted species reactivity
Mouse
Immunogen
Synthetic peptide within Human KCNA1 aa 491-440 / 495.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:2, 000-1:5, 000
; IHC-P: 1:50-1:200
; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 56 kDa
Subcellular location
Cell membrane, Membrane, Cytoplasmic vesicle, Perikaryon, Endoplasmic reticulum.
Positive control
Human brain tissue lysates, rat brain tissue.