![AffiAB® Anti-Ku70 Antibody [D1-A6]](/web/image/product.product/76784/image_1024/%5BAFG-HB-2266%5D%20AffiAB%C2%AE%20Anti-Ku70%20Antibody%20%5BD1-A6%5D%20?unique=8544d55)
AffiAB® Anti-Ku70 Antibody [D1-A6]
Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V (D) J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system.In addition to its role in NHEJ, Ku is also required for telomere length maintenance and subtelomeric gene silencing. Ku was originally identified when patients with systemic lupus erythematosus were found to have high levels of autoantibodies to the protein.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: P12956 Human; SwissProt: P23475 mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG2a
Clone
D1-A6
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human Ku70 aa 1-150/609.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:2, 000
; IHC-P: 1:100-1:200
Molecular Weight
Predicted band size: 70 kDa
Subcellular location
Nucleus.
Positive control
Hela cell lysates, HepG2 cell lysates, A431 cell lysates, D3 cell lysates, 293T cell lysates, A549 cell lysates, COS-1 cell lysates, human kidney tissue lysates, human liver tissue lysates, human brain tissue lysates, human thymus tissue lysates, human placentae tissue lysates, mouse kidney tissue lysates, mouse liver tissue lysates, mouse thymus tissue lysates, mouse testis tissue lysates, human tonsil tissue, human breast cancer tissue.