AffiAB® Anti-KLF4 Antibody [JF98-08]
The Kruppel-type zinc finger transcription factors comprise a conserved family of DNA binding proteins that are important in developmental regulation. The Kruppel zinc finger transcription factor was initially identified in Drosophila as a segmentation gene. Kruppel-like factors that have been characterized in mammals include EKLF, LKLF and GKLF (6-8) . EKLF is expressed principally in erythroid tissues, and LKLF expression is limited to the lung. GKLF is found predominantly in gut and has been shown to be expressed during growth arres.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: O43474 Human; SwissProt: Q60793 Mouse; Unigene: 7719 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JF98-08
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P
Predicted species reactivity
Rat
Immunogen
Synthetic peptide within human KLF4 aa 320-360.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200
Molecular Weight
55 kDa
Subcellular location
Nucleus.
Positive control
293 cell lysate, NCCIT cell lysate, Hela, PC-3M, 293T, human stomach carcinoma tissue, mouse colon tissue, mouse stomach tissue, human liver tissue, mouse liver tissue.