![AffiAB® Anti-KIF5B Antibody [JE64-16]](/web/image/product.product/76757/image_1024/%5BAFG-HB-2239%5D%20AffiAB%C2%AE%20Anti-KIF5B%20Antibody%20%5BJE64-16%5D%20?unique=b83e256)
AffiAB® Anti-KIF5B Antibody [JE64-16]
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation. Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends. Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P33176 Human; SwissProt: Q61768 Mouse; SwissProt: Q2PQA9 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JE64-16
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human KIF5B aa 1-50.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500
; IHC-P: 1:100-1:400
Molecular Weight
Predicted band size: 110 kDa
Subcellular location
Cytolytic granule membrane, Lysosome membrane, cytoskeleton.
Positive control
HeLa cell lysate, A549 cell lysate, mouse kidney tissue lysate, HCT116 cell lysate, C2C12 cell lysate, MCF-7 cell lysate, Hela cell lysate, HepG2 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human gallbladder tissue, human testis tissue, rat uterus tissue, rat cerebellum tissue lysate, rat bladder tissue lysate.