AffiAB® Anti-KIF3A Antibody
Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitement of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q9Y496 Human; SwissProt: P28741 Mouse; Unigene: 47400 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human KIF3A aa 154-364 / 699.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:1, 000
; IHC-P: 1:50-1:100
; FC: 1:50-1:100
Molecular Weight
Predicted band size: 80 kDa
Subcellular location
Cytoskeleton.
Positive control
Rat testis tissue, Daudi, rat cerebellum tissue, mouse testis tissue, A549.