AffiAB® Anti-Ki67 Antibody [ST50-01]
Ki-67 is a nuclear protein that is expressed in proliferating cells and may be required for maintaining cell proliferation. Ki-67 has been used as a marker for cell proliferation of solid tumors and some hematological malignancies. A correlation has been demonstrated between Ki-67 index and the histopathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P46013 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
ST50-01
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human Ki67 aa 1040-1080.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Cancer, Neuroscience
Recommended dilutions
WB: 1:1, 000-1:2, 000; IF-Cell: 1:50-1:1, 000; IHC-P: 1:100-1:500; FC: 1:50-1:100
Molecular Weight
Predicted band size: 359 kDa
Subcellular location
Nucleus, Chromosome.
Positive control
HepG2 cell lysates, HepG2, human tonsil tissue, human stomach carcinoma tissue, human colon carcinoma tissue, Hela.