![AffiAB® Anti-Insulin degrading enzyme Antibody [JJ0949]](/web/image/product.product/74886/image_1024/%5BAFG-HB-2113%5D%20AffiAB%C2%AE%20Anti-Insulin%20degrading%20enzyme%20Antibody%20%5BJJ0949%5D%20?unique=d31c23b)
AffiAB® Anti-Insulin degrading enzyme Antibody [JJ0949]
Insulin degrading enzyme (IDE) , also known as insulinase, insulin protease or insulysin, cleaves the peptide hormone insulin with consequences for insulin response and resistance. IDE is a highly conserved thiol metalloprotease with ubiquitous expression including the brain. Since its discovery, IDE has been shown to degrade a variety of bioactive peptides. Recently the finding that IDE clears intracellular and extracellular amyloid products has spurred research into the link between IDE function and human neurodegenerative disease such as Alzheimer’s.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P14735 Human; SwissProt: Q9JHR7 Mouse; SwissProt: P35559 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JJ0949
KO/KD
N/A
Species reactivity
Human, Mouse, Rat, Zebrafish
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human Insulin degrading enzyme aa 1-106 / 1019.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:2, 000
; IHC-P: 1:50-1:200
Molecular Weight
118 kDa
Subcellular location
Cytoplasm, Cell membrane, Secreted.
Positive control
Zebrafish tissue lysate, Hela cell lysate, K562 cell lysate, human liver carcinoma tissue, human colon carcinoma tissue, human stomach carcinoma tissue, mouse colon tissue, hybrid fish (crucian-carp) heart tissue lysates.