AffiAB® Anti-Insulin Antibody [A6-6]
Insulin is a hormone with extensive effects on both metabolism and several other body systems. It causes most of the body's cells to take up glucose from the blood (including liver, muscle, and fat tissue cells) , storing it as glycogen in the liver and muscle, and stops use of fat as an energy source. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: P01308 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG1
Clone
A6-6
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
IHC-P, ELISA
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human Insulin aa 25-54.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
HIF-1 signaling pathway, PI3K-AKT, cGMP-PKG signaling pathway, Type II diabetes mellitus, Immunology & Inflammation, Autophagy Alzheimer disease, AMPK signaling pathway
Recommended dilutions
IHC-P: 1:100-1:1, 000
; ELISA: 1:10, 000
Molecular Weight
Predicted band size: 12 kDa
Subcellular location
Secreted.
Positive control
Rat pancreas tissue, human pancreas tissue, mouse pancreas tissue.