AffiAB® Anti-IL-10RA Antibody
Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q13651 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IHC-P, IF-Cell
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human IL-10RA aa 491-540/578.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
PBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IHC-P: 1:200-1:1, 000; IF-Cell: 1:50-1:200
Molecular Weight
63 kDa
Subcellular location
Cytoplasm, Cell membrane.
Positive control
Daudi cell lysates, Jurkat cell lysate, THP-1 cell lysate, MCF-7 cell lysate, human tonsil tissue, SiHa.