AffiAB® Anti-IL-1 beta Antibody [JJ087-3]
Two forms of interleukin-1, designated IL-1α and IL-1β, have been described. Although encoded by distinct genes and exhibiting roughly only 25% sequence identity, IL-1α and IL-1β bind to the same receptor and seem to elicit similar biological responses. IL-1 production is generally thought to be associated with inflammation, but it has also been shown to be expressed during kidney development, thymocyte differentiation and cartilage degradation. IL-1 plays a critical role in the regulation of immune response and inflammation, acting as an activator of T and B lymphocytes and natural killer (NK) cells. In T cells, IL-1 stimulates the production of IL-2 and selectively inhibits IL-4 expression. IL-1 induces B cell proliferation and maturation, and immunoglobulin synthesis. NK cells require IL-1β for production of the anti- pathogen IFN-γ. IL-1 has also been implicated in several pathological conditions including rheumatoid arthritis, inflammatory bowel disease and atherosclerosis.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P01584 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JJ087-3
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within C-terminal human IL1 beta.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
细胞焦亡, NF-KB signaling pathway, Rheumatoid arthritis, Immunology & Inflammation, 细胞因子, Alzheimer disease, MAPK signaling pathway
Recommended dilutions
WB: 1:1, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
Predicted band size: 31 kDa
Subcellular location
Cytoplasm, Lysosome, extracellular exosome, Secreted.
Positive control
A431, Hela, human kidney tissue.