![AffiAB® Anti-IKB alpha Antibody [SZ00-07]](/web/image/product.product/74792/image_1024/%5BAFG-HB-2014%5D%20AffiAB%C2%AE%20Anti-IKB%20alpha%20Antibody%20%5BSZ00-07%5D%20?unique=a48b62f)
AffiAB® Anti-IKB alpha Antibody [SZ00-07]
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P25963 Human; SwissProt: Q9Z1E3 Mouse; SwissProt: Q63746 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SZ00-07
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human IKB alpha aa 1-50.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Apotosis, cAMP signaling pathway, Immunology & Inflammation, NF-KB signaling pathway
Recommended dilutions
WB: 1:500-1:5, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200; IP: Use at an assay dependent concentration.
Molecular Weight
36 kDa
Subcellular location
Cytoplasm, Nucleus.
Positive control
NIH/3T3 tissue lysate, PC-12 tissue lysate, Hela tissue lysate, Hela, MCF-7, SHG-44, human kidney tissue, mouse kidney tissue, mouse brain tissue, mouse stomach tissue.