AffiAB® Anti-Iba1 Antibody
Ionized calcium-binding adapter molecule 1 (Iba1) , also known as allograft inflammatory factor-1 (AIF-1) , is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC) .
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P55008 Human; SwissProt: O70200 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within C-terminal human Iba1.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Neuroscience
Recommended dilutions
IHC-P: 1:50-1:200
Molecular Weight
17/10/15 kDa
Subcellular location
Plasma membrane. Cytoskeleton.
Positive control
Human tonsil tissue, human spleen tissue, mouse spleen tissue.