![AffiAB® Anti-HSD11B1 Antibody [JU18-39]](/web/image/product.product/74714/image_1024/%5BAFG-HB-1933%5D%20AffiAB%C2%AE%20Anti-HSD11B1%20Antibody%20%5BJU18-39%5D%20?unique=a48b62f)
AffiAB® Anti-HSD11B1 Antibody [JU18-39]
HSD11B1 (Corticosteroid 11-beta-dehydrogenase isozyme 1) is also named as HSD11, HSD11L, 11-DH and 11-beta-HSD1.It is a microsomal enzyme, which acts mainly as a NADP (H) - dependent reductase converting inactive cortisone (E) to active cortisol (F) , thus regulating intracellular F access to glucocorticoid receptor. This enzyme is widely expressed not only in liver and adipose tissue, but also in adrenal, ovary and decidua.Western blot analysis in human liver, using a specific11-beta-HSD1 antibody, showed two bands at approximately 34 and 68 kDa confrming the dimeric nature of 11-beta-HSD1 in liver and In omental adipose tissue, an additional band of 50 kDa was strongly expressed.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P28845 Human; SwissProt: P50172 Mouse; SwissProt: P16232 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JU18-39
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human HSD11B1 aa 1-50 / 292.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000
; IHC-P: 1:50-1:200
Molecular Weight
32 kDa
Subcellular location
Endoplasmic reticulum, Membrane.
Positive control
Mouse liver tissue lysates, human liver tissue, mouse liver tissue.