![AffiAB® Anti-HP1 alpha Antibody [SR44-03]](/web/image/product.product/74708/image_1024/%5BAFG-HB-1926%5D%20AffiAB%C2%AE%20Anti-HP1%20alpha%20Antibody%20%5BSR44-03%5D%20?unique=a48b62f)
AffiAB® Anti-HP1 alpha Antibody [SR44-03]
The family of Heterochromatin Protein 1 (HP1) ("Chromobox Homolog", CBX) are highly conserved adapter proteins, which have important functions in the cell nucleus. These functions include gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.Three different paralogs of HP1 are found in mammals, HP1α, HP1βand HP1γ.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P45973 Human; SwissProt: Q61686 Mouse; Entrez Gene: 300266 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SR44-03
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human HP1 alpha aa 101-191 / 191.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 22 kDa
Subcellular location
Nucleus, Chromosome
Positive control
MCF-7 cell lysate, Hela cell lysate, Hela, MCF-7, human tonsil tissue, human breast carcinoma tissue, human uterus tissue, mouse uterus tissue, SH-SY5Y, rat uterus tissue.