AffiAB® Anti-HP1 alpha Antibody [B0-1]
Chromobox protein homolog 5 is a protein that in humans is encoded by the CBX5 gene. It is a highly conserved, non-histone protein part of the heterochromatin family. The protein itself is more commonly called (in humans) HP1α. Heterochromatin protein-1 (HP1) has an N-terminal domain that acts on methylated lysines residues leading to epigenetic repression. The C-terminal of this protein has a chromo shadow-domain (CSD) that is responsible for homodimerizing, as well as interacting with a variety of chromatin-associated, non-histone proteins. HP1α is a highly evolutionary conserved protein, existing in species such a Schizosaccharomyces pombe, a type of yeast, all the way to humans. The N-terminal chromodomain and C-terminal chromoshadow domain appear to be much more conserved (approximately 50-70% amino acid similarity) than the hinge region (approximately 25-30% similarity with the Drosophila HP1 homolog) .
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: P45973 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG1
Clone
B0-1
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell
Predicted species reactivity
Mouse
Immunogen
Recombinant protein within Human HP1 alpha aa 1-191 / 191.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:5, 000; IF-Cell: 1:500
Molecular Weight
Predicted band size: 22 kDa
Subcellular location
Nucleus
Positive control
MCF-7 cell lysates, MCF-7.