AffiAB® Anti-HNF1 alpha Antibody
The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P20823 Human; SwissProt: P22361 Mouse; SwissProt: P15257 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human HNF1 alpha aa 100-300 / 631.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:5, 000
; IHC-P: 1:100-1:500
; FC: 1:500-1:1, 000
Molecular Weight
67 kDa
Subcellular location
Nucleus.
Positive control
HepG2 cell lysate, mouse uterus tissue lysate, human kidney tissue lysate, rat kidney tissue, human small intestine tissue, mouse liver tissue, HepG2.